Investigation of the antioxidant status and the number of double-stranded DNA breaks in models of brain tumor lesion by metastases of non-small cell lung cancer in vivo

Purpose of the study . Evaluation antioxidant status and DNA damage in tissues subcutaneous xenografts non-small cell lung cancer peritumoral created using A549 H1299 cultures. Materials methods The included 35 intact male Balb/c Nude immunodeficient mice. Cell lines were used as transplantable tumor biomaterial. A CDX model was accordance with protocol for supratentorial injections (Ozawa T., James C. D., 2010) adapted this experiment. Growth rates controlled intracranial visualized a high-resolution micro-CT system. activity catalase superoxide dismutase determined non-denaturing electrophoresis 8 % 12 polyacrylamide gel. concentrations sulfhydryl groups according to Ellman. lymphocytes by comet assay. Results experiment resulted creation models brain tumors characterized growth pattern 100 %. studied lysates xenografts, tissue healthy tumor-bearing animals all experimental increased statistically significantly relative animals, greatest differences from control recorded group implanted culture at concentration 1 × 106 Superoxide compared sample groups. highest increase SOD observed load, which amounted 28.8 32.9 changes sample. significant SH-groups revealed groups, (36.2 ± 0.47) load. Percentage change tail moment (DNA indicator) O1, O2, O3 O4 55.8 %, 111.8 97.3 170 respectively. Conclusions defense system, accumulation oxidative modifications proteins, an double-strand breaks vivo suggest that reflect processes similar those human cancer.